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KMID : 0358419660090050013
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Korean Journal of Obstetrics and Gynecology
1966 Volume.9 No. 5 p.13 ~ p.16
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The Effect of Estradiol-17 on the Activity of Uterine Carbamyl phosphate
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任沅柱/Im, W.J.
李重培/林弘正/Lee, J.B./Im, H.J.
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Abstract
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The changes induced by the administration of estradiol-17 in the activity of carbamyl phosphate: L-aspartate transcarbamylase in the uteri of immature rats were observed.
A total of 10 ?g estradiol-17 administered intraperitoneally in divided doses, caused a gradual rise in ´the enzyme activity, reaching a maximum value of more than two-fold increase over controls around 30 to 40 hours after the hormone administration. Thereafter the enzyme activity fell to control values again.
A colorimetnc method for the assay off the enzyme activity is presented, which is consisted essentially of precipitating inorganc phosphate liberated from carbamyl phosphate as itriethylamine-phosphomolybdate complex and then colorizing with stannous chloride.
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